An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

System-Wide Modulation of HECT E3 Ligases with Selective Ubiquitin Variant Probes.

Zhang W, Wu KP, Sartori MA, Kamadurai HB, Ordureau A, Jiang C, Mercredi PY, Murchie R, Hu J, Persaud A, Mukherjee M, Li N, Doye A, Walker JR, Sheng Y, Hao Z, Li Y, Brown KR, Lemichez E, Chen J, Tong Y, Harper JW, Moffat J, Rotin D, Schulman BA, Sidhu SS

HECT-family E3 ligases ubiquitinate protein substrates to control virtually every eukaryotic processÂ and are misregulated in numerous diseases. Nonetheless, understanding of HECT E3s is limited byÂ aÂ paucity of selective and potent modulators. ToÂ overcome this challenge, we systematically developed ubiquitin variants (UbVs) that inhibit or activate HECT E3s. Structural analysis of 6 HECT-UbV complexes revealed UbV inhibitors hijacking the E2-binding site and activators ... [more]

Mol. Cell Apr. 07, 2016; 62(1);121-36 [Pubmed: 26949039]

Throughput

Low Throughput

Additional Notes

auto-ubiquitination assay

Curated By

BioGRID

Interaction Summary

HECW2

Gene Ontology Biological Process

Gene Ontology Molecular Function

ubiquitin-protein transferase activity [IBA]

Gene Ontology Cellular Component