An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase Î².

Fang Q, Inanc B, Schamus S, Wang XH, Wei L, Brown AR, Svilar D, Sugrue KF, Goellner EM, Zeng X, Yates NA, Lan L, Vens C, Sobol RW

Cellular DNA repair processes are crucial to maintain genome stability and integrity. In DNA base excision repair, a tight heterodimer complex formed by DNA polymerase Î² (PolÎ²) and XRCC1 is thought to facilitate repair by recruiting PolÎ² to DNA damage sites. Here we show that disruption of the complex does not impact DNA damage response or DNA repair. Instead, the ... [more]

Nat Commun Nov. 27, 2014; 5(0);5513 [Pubmed: 25423885]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
XRCC1 POLB	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Hein MY (2015)	High	-	BioGRID	1446185
POLB XRCC1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2015)	High	1	BioGRID	1188355
POLB XRCC1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2017)	High	1	BioGRID	2218061
POLB XRCC1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	1	BioGRID	3041973
XRCC1 POLB	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Liu X (2021)	Low	-	BioGRID	-
POLB XRCC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Bhattacharyya N (2001)	Low	-	BioGRID	-
POLB XRCC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Fang Q (2014)	Low	-	BioGRID	-
XRCC1 POLB	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Hu LY (2018)	Low	-	BioGRID	-
XRCC1 POLB	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Liu X (2021)	Low	-	BioGRID	-
XRCC1 POLB	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Luo H (2004)	Low	-	BioGRID	-
XRCC1 POLB	Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe.	Hu LY (2018)	Low	-	BioGRID	-
XRCC1 POLB	Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe.	Kubota Y (1996)	Low	-	BioGRID	-
POLB XRCC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Bhattacharyya N (2001)	Low	-	BioGRID	-
XRCC1 POLB	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Fan J (2004)	Low	-	BioGRID	-
XRCC1 POLB	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Hu LY (2018)	Low	-	BioGRID	-
POLB XRCC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Hu LY (2018)	Low	-	BioGRID	-
XRCC1 POLB	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Kubota Y (1996)	Low	-	BioGRID	-
XRCC1 POLB	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Taylor RM (2002)	Low	-	BioGRID	-
POLB XRCC1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Wang L (2004)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

XRCC1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

POLB