An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

FBG1 Is the Final Arbitrator of A1AT-Z Degradation.

Wen JH, Wen H, Gibson-Corley KN, Glenn KA

Alpha-1 antitrypsin deficiency is the leading cause of childhood liver failure and one of the most common lethal genetic diseases. The disease-causing mutant A1AT-Z fails to fold correctly and accumulates in the endoplasmic reticulum (ER) of the liver, resulting in hepatic fibrosis and hepatocellular carcinoma in a subset of patients. Furthermore, A1AT-Z sequestration in hepatocytes leads to a reduction in ... [more]

PLoS ONE Aug. 22, 2015; 10(8);e0135591 [Pubmed: 26295339]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

FBXO2

Gene Ontology Biological Process

Gene Ontology Molecular Function

ubiquitin-protein transferase activity [TAS]

Gene Ontology Cellular Component

SERPINA1