USP2
Gene Ontology Biological Process
- circadian behavior [ISS]
- circadian regulation of gene expression [ISS]
- entrainment of circadian clock by photoperiod [ISS]
- locomotor rhythm [ISS]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of mitotic cell cycle [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein deubiquitination [IDA]
- protein stabilization [IDA, IMP]
- regulation of proteasomal protein catabolic process [IBA]
Gene Ontology Molecular Function
TRAF2
Gene Ontology Biological Process
- activation of NF-kappaB-inducing kinase activity [IMP]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [TAS]
- apoptotic process [TAS]
- apoptotic signaling pathway [TAS]
- cellular protein complex assembly [ISS]
- innate immune response [TAS]
- negative regulation of neuron death [TAS]
- positive regulation of JUN kinase activity [IDA]
- positive regulation of NF-kappaB transcription factor activity [IDA, IMP]
- positive regulation of T cell activation [IC]
- positive regulation of T cell cytokine production [IMP]
- positive regulation of extrinsic apoptotic signaling pathway [IMP]
- positive regulation of interleukin-2 production [IMP]
- positive regulation of protein homodimerization activity [IMP]
- positive regulation of sequence-specific DNA binding transcription factor activity [IMP]
- protein K63-linked ubiquitination [IDA]
- protein autoubiquitination [IDA, TAS]
- protein complex assembly [TAS]
- protein homotrimerization [IPI]
- regulation of apoptotic process [IDA]
- regulation of extrinsic apoptotic signaling pathway in absence of ligand [TAS]
- signal transduction [TAS]
- tumor necrosis factor-mediated signaling pathway [IDA]
Gene Ontology Molecular Function- CD40 receptor binding [ISS]
- enzyme binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- protein phosphatase binding [IPI]
- signal transducer activity [NAS]
- sphingolipid binding [IDA]
- thioesterase binding [IPI]
- tumor necrosis factor receptor binding [IPI]
- ubiquitin protein ligase binding [IPI]
- ubiquitin-protein transferase activity [IDA]
- CD40 receptor binding [ISS]
- enzyme binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- protein phosphatase binding [IPI]
- signal transducer activity [NAS]
- sphingolipid binding [IDA]
- thioesterase binding [IPI]
- tumor necrosis factor receptor binding [IPI]
- ubiquitin protein ligase binding [IPI]
- ubiquitin-protein transferase activity [IDA]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
De-ubiquitinating proteases USP2a and USP2c cause apoptosis by stabilising RIP1.
Dynamic ubiquitination impacts on the degradation of proteins by the proteasome as well as on their effects as signalling factors. Of the many cellular responses that are regulated by changes in ubiquitination, apoptosis has garnered special attention. We have found that USP2a and USP2c, two isoforms of the ubiquitin-specific protease USP2, cause cell death upon ectopic expression. We show that ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| USP2 TRAF2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| TRAF2 USP2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| USP2 TRAF2 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 605125 | |
| TRAF2 USP2 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| USP2 TRAF2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 2695979 | |
| TRAF2 USP2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - | |
| TRAF2 USP2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID