BAIT

KLF5

BTEB2, CKLF, IKLF, RP11-505F3.5
Kruppel-like factor 5 (intestinal)
Homo sapiens
PREY

OGT

HRNT1, O-GLCNAC
O-linked N-acetylglucosamine (GlcNAc) transferase
Alzheimer's Disease Project
GO Process (24)
GO Function (9)
GO Component (8)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

BAP1 promotes breast cancer cell proliferation and metastasis by deubiquitinating KLF5.

Qin J, Zhou Z, Chen W, Wang C, Zhang H, Ge G, Shao M, You D, Fan Z, Xia H, Liu R, Chen C

The transcription factor KLF5 is highly expressed in basal-like breast cancer and promotes breast cancer cell proliferation, survival, migration and tumour growth. Here we show that, in breast cancer cells, KLF5 is stabilized by the deubiquitinase (DUB) BAP1. With a genome-wide siRNA library screen of DUBs, we identify BAP1 as a bona fide KLF5 DUB. BAP1 interacts directly with KLF5 ... [more]

Nat Commun Oct. 01, 2015; 6(0);8471 [Pubmed: 26419610]

Throughput

  • Low Throughput

Curated By

  • BioGRID