BAIT

EZH2

Enx-1, Enx1h, KMT6, mKIAA4065
enhancer of zeste homolog 2 (Drosophila)
GO Process (29)
GO Function (9)
GO Component (6)
Mus musculus
PREY

MDM2

1700007J15Rik, AA415488, Mdm-2
transformed mouse 3T3 cell double minute 2
GO Process (24)
GO Function (9)
GO Component (9)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

MDM2 Associates with Polycomb Repressor Complex 2 and Enhances Stemness-Promoting Chromatin Modifications Independent of p53.

Wienken M, Dickmanns A, Nemajerova A, Kramer D, Najafova Z, Weiss M, Karpiuk O, Kassem M, Zhang Y, Lozano G, Johnsen SA, Moll UM, Zhang X, Dobbelstein M

The MDM2 oncoprotein ubiquitinates and antagonizes p53 but may also carry out p53-independent functions. Here we report that MDM2 is required for the efficient generation of induced pluripotent stem cells (iPSCs) from murine embryonic fibroblasts, in the absence of p53. Similarly, MDM2 depletion in the context of p53 deficiency also promoted the differentiation of human mesenchymal stem cells and diminished ... [more]

Mol. Cell Jan. 07, 2016; 61(1);68-83 [Pubmed: 26748827]

Throughput

  • Low Throughput

Curated By

  • BioGRID