BAIT

PRP19

PSO4, E3 ubiquitin-protein ligase PRP19, L000001506, YLL036C
Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain; relocalizes to the cytosol in response to hypoxia
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

PRP39

L000001511, YML046W
U1 snRNP protein involved in splicing; contains multiple tetriatricopeptide repeats
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

Composition and functional characterization of the yeast spliceosomal penta-snRNP.

Stevens SW, Ryan DE, Ge HY, Moore RE, Young MK, Lee TD, Abelson J

Pre-mRNA introns are spliced in a macromolecular machine, the spliceosome. For each round of splicing, the spliceosome assembles de novo in a series of ATP-dependent steps involving numerous changes in RNA-RNA and RNA-protein interactions. As currently understood, spliceosome assembly proceeds by addition of discrete U1, U2, and U4/U6*U5 snRNPs to a pre-mRNA substrate to form functional splicing complexes. We characterized ... [more]

Mol. Cell Jan. 01, 2002; 9(1);31-44 [Pubmed: 11804584]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PRP39 PRP19
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1568BioGRID
1945874

Curated By

  • BioGRID