NDUFS3
Gene Ontology Biological Process
- cellular metabolic process [TAS]
- mitochondrial electron transport, NADH to ubiquinone [NAS]
- negative regulation of cell growth [IMP]
- negative regulation of intrinsic apoptotic signaling pathway [IMP]
- reactive oxygen species metabolic process [IMP]
- respiratory electron transport chain [TAS]
- small molecule metabolic process [TAS]
- substantia nigra development [IEP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
NDUFA5
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function.
Mitochondria are essential for numerous cellular processes, yet hundreds of their proteins lack robust functional annotation. To reveal functions for these proteins (termed MXPs), we assessed condition-specific protein-protein interactions for 50 select MXPs using affinity enrichment mass spectrometry. Our data connect MXPs to diverse mitochondrial processes, including multiple aspects of respiratory chain function. Building upon these observations, we validated C17orf89 ... [more]
Quantitative Score
- 99.0 [Confidence Score]
Throughput
- High Throughput
Additional Notes
- The scores represent percentile of the interaction (out of all interactions) based on the CompPASS algorithm.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NDUFS3 NDUFA5 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| NDUFS3 NDUFA5 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9853 | BioGRID | 1178980 | |
| NDUFS3 NDUFA5 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9592 | BioGRID | 2250047 | |
| NDUFS3 NDUFA5 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.957 | BioGRID | 3035188 | |
| NDUFS3 NDUFA5 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9996 | BioGRID | 3284629 | |
| NDUFA5 NDUFS3 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.88 | BioGRID | 743953 | |
| NDUFA5 NDUFS3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 2721749 |
Curated By
- BioGRID