An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function.

Floyd BJ, Wilkerson EM, Veling MT, Minogue CE, Xia C, Beebe ET, Wrobel RL, Cho H, Kremer LS, Alston CL, Gromek KA, Dolan BK, Ulbrich A, Stefely JA, Bohl SL, Werner KM, Jochem A, Westphall MS, Rensvold JW, Taylor RW, Prokisch H, Kim JP, Coon JJ, Pagliarini DJ

Mitochondria are essential for numerous cellular processes, yet hundreds of their proteins lack robust functional annotation. To reveal functions for these proteins (termed MXPs), we assessed condition-specific protein-protein interactions for 50 select MXPs using affinity enrichment mass spectrometry. Our data connect MXPs to diverse mitochondrial processes, including multiple aspects of respiratory chain function. Building upon these observations, we validated C17orf89 ... [more]

Mol. Cell Aug. 03, 2016; 0(0); [Pubmed: 27499296]

Quantitative Score

98.2 [Confidence Score]

Throughput

High Throughput

Additional Notes

The scores represent percentile of the interaction (out of all interactions) based on the CompPASS algorithm.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NDUFS3 NDUFB11	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Guarani V (2014)	Low	-	BioGRID	-
NDUFB11 NDUFS3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2017)	High	0.8711	BioGRID	2262039
NDUFB11 NDUFS3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.8816	BioGRID	3103180

Curated By

BioGRID

Interaction Summary

NDUFS3

Gene Ontology Biological Process

Gene Ontology Molecular Function

NADH dehydrogenase (ubiquinone) activity [NAS]NADH dehydrogenase activity [IMP]electron carrier activity [NAS]protein binding [IPI]

Gene Ontology Cellular Component

NDUFB11