BAIT

PRP19

PSO4, E3 ubiquitin-protein ligase PRP19, L000001506, YLL036C

Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain; relocalizes to the cytosol in response to hypoxia

UPS Project

GO Process (1)

GO Function (2)

GO Component (6)

Gene Ontology Biological Process

generation of catalytic spliceosome for first transesterification step [IMP]

Gene Ontology Molecular Function

first spliceosomal transesterification activity [IC]
ubiquitin-protein transferase activity [IDA, IMP]

Gene Ontology Cellular Component

Prp19 complex [IDA]

U2-type catalytic step 1 spliceosome [IDA]

cytoplasm [IDA]

cytosol [IDA]

mitochondrion [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SWI4

ART1, SBF complex DNA-binding subunit SWI4, L000000124, L000002252, YER111C

DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; Slt2p-independent regulator of cold growth; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p

GO Process (2)

GO Function (2)

GO Component (2)

Gene Ontology Biological Process

positive regulation of transcription from RNA polymerase II promoter in response to heat stress [IMP]

positive regulation of transcription involved in G1/S transition of mitotic cell cycle [IMP]

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

SBF transcription complex [IDA, IMP, IPI]

nuclear chromatin [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

Composition and functional characterization of the yeast spliceosomal penta-snRNP.

Stevens SW, Ryan DE, Ge HY, Moore RE, Young MK, Lee TD, Abelson J

Pre-mRNA introns are spliced in a macromolecular machine, the spliceosome. For each round of splicing, the spliceosome assembles de novo in a series of ATP-dependent steps involving numerous changes in RNA-RNA and RNA-protein interactions. As currently understood, spliceosome assembly proceeds by addition of discrete U1, U2, and U4/U6*U5 snRNPs to a pre-mRNA substrate to form functional splicing complexes. We characterized ... [more]

Mol. Cell Jan. 01, 2002; 9(1);31-44 [Pubmed: 11804584]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SWI4 PRP19	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.2155	BioGRID	2040524

Curated By

BioGRID

Interaction Summary

PRP19

Gene Ontology Biological Process

Gene Ontology Molecular Function

first spliceosomal transesterification activity [IC]ubiquitin-protein transferase activity [IDA, IMP]

Gene Ontology Cellular Component

SWI4

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

Co-purification

Publication

Composition and functional characterization of the yeast spliceosomal penta-snRNP.

Throughput

Related interactions

Curated By

first spliceosomal transesterification activity [IC]
ubiquitin-protein transferase activity [IDA, IMP]

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
sequence-specific DNA binding [IDA]