Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function.
Mitochondria are essential for numerous cellular processes, yet hundreds of their proteins lack robust functional annotation. To reveal functions for these proteins (termed MXPs), we assessed condition-specific protein-protein interactions for 50 select MXPs using affinity enrichment mass spectrometry. Our data connect MXPs to diverse mitochondrial processes, including multiple aspects of respiratory chain function. Building upon these observations, we validated C17orf89 ... [more]
Mol. Cell Aug. 03, 2016; 0(0); [Pubmed: 27499296]
Quantitative Score
- 96.0 [Confidence Score]
Throughput
- High Throughput
Additional Notes
- The scores represent percentile of the interaction (out of all interactions) based on the CompPASS algorithm.
Curated By
- BioGRID