An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Glutaredoxin GRXS17 Associates with the Cytosolic Iron-Sulfur Cluster Assembly Pathway.

Inigo S, Nagels Durand A, Ritter A, Le Gall S, Termathe M, Klassen R, Tohge T, De Coninck B, Van Leene J, De Clercq R, Cammue B, Fernie AR, Gevaert K, De Jaeger G, Leidel SA, Schaffrath R, Van Lijsebettens M, Pauwels L, Goossens A

Cytosolic monothiol glutaredoxins (GRXs) are required in iron-sulfur (Fe-S) cluster delivery and iron sensing in yeast and mammals. In plants, it is unclear whether they have similar functions. Arabidopsis thaliana (Arabidopsis) has a sole class II cytosolic monothiol GRX encoded by GRXS17. Here, we used tandem affinity purification to establish that Arabidopsis GRXS17 associates with most known cytosolic Fe-S assembly ... [more]

Plant Physiol. Aug. 08, 2016; 0(0); [Pubmed: 27503603]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AT4G04950 AT5G09830	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Braun P (2011)	High	-	BioGRID	-
AT4G04950 AT5G09830	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Couturier J (2013)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

AT4G04950

Gene Ontology Biological Process

Gene Ontology Cellular Component

AT5G09830

Gene Ontology Molecular Function

protein binding [IPI]