BAIT

DTL

CDT2, DCAF2, L2DTL, RAMP, RP11-15I11.1
denticleless E3 ubiquitin protein ligase homolog (Drosophila)
UPS Project
Homo sapiens
PREY

RBX1

BA554C12.1, RNF75, ROC1, RP11-554C12.1
ring-box 1, E3 ubiquitin protein ligase
UPS Project
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Selective ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G ubiquitin-conjugating enzymes via the CRL4Cdt2 ubiquitin ligase complex.

Shibata E, Abbas T, Huang X, Wohlschlegel JA, Dutta A

CRL4(Cdt2) is a cullin-based E3 ubiquitin ligase that promotes the ubiquitin-dependent proteolysis of various substrates implicated in the control of cell cycle and various DNA metabolic processes such as DNA replication and repair. Substrates for CRL4(Cdt2) E3 ubiquitin ligase include the replication licensing factor Cdt1 and the cyclin-dependent kinase (Cdk) inhibitor p21. Inhibition of this E3 ligase leads to serious ... [more]

Mol. Cell. Biol. Aug. 01, 2011; 31(15);3136-45 [Pubmed: 21628527]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DTL RBX1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID