BAIT

GRB2

ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2
growth factor receptor-bound protein 2
Homo sapiens
PREY

LIME1

LIME, LP8067, dJ583P15.4, RP4-583P15.5
Lck interacting transmembrane adaptor 1
GO Process (0)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Non-T cell activation linker regulates ERK activation in Helicobacter pylori-infected epithelial cells.

Rieke C, Kaehne T, Schweitzer K, Schraven B, Wienands J, Engelke M, Naumann M

It is supposed that human pathogens, e.g. Helicobacter pylori abuse lipid raft domains on the host cell plasma membrane to infect the cell. Investigating DRM-associated molecules we identified the transmembrane adapter proteins (TRAPs), non-T cell activation linker (NTAL) and lymphocyte-specific protein tyrosine kinase (Lck)-interacting membrane protein (LIME) to be regulated by H. pylori in the human epithelial cell line HCA-7. ... [more]

Cell. Signal. Mar. 01, 2010; 22(3);395-403 [Pubmed: 19879355]

Throughput

  • Low Throughput

Additional Notes

  • Figure 4

Curated By

  • BioGRID