BAIT

PTGS2

COX2, Cox-2, PGHS-2, PHS-2, Pghs2, TIS10
prostaglandin-endoperoxide synthase 2
GO Process (42)
GO Function (6)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

NOS1

2310005C01Rik, NO, NOS-I, Nos-1, bNOS, nNOS
nitric oxide synthase 1, neuronal
GO Process (40)
GO Function (15)
GO Component (20)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

S-nitrosylation/activation of COX-2 mediates NMDA neurotoxicity.

Tian J, Kim SF, Hester L, Snyder SH

Glutamate/N-methyl-d-aspartate (NMDA) receptor-mediated neurotoxicity involves cyclooxygenase (COX)-2. We demonstrate that this neurotoxicity reflects activation of COX-2 by S-nitrosylation after selective binding of neuronal nitric oxide synthase (nNOS) to COX-2. nNOS, via its PDZ domain, binds COX-2 with the generated NO S-nitrosylating and activating the enzyme. Selective disruption of nNOS-COX-2 binding prevents NMDA neurotoxicity. ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jul. 29, 2008; 105(30);10537-40 [Pubmed: 18650379]

Throughput

  • Low Throughput

Additional Notes

  • Figure 1

Curated By

  • BioGRID