BAIT

ZFP36L1

BRF1, Berg36, ERF-1, ERF1, RNF162B, TIS11B, cMG1
ZFP36 ring finger protein-like 1
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The AU-rich element mRNA decay-promoting activity of BRF1 is regulated by mitogen-activated protein kinase-activated protein kinase 2.

Maitra S, Chou CF, Luber CA, Lee KY, Mann M, Chen CY

Regulated mRNA decay is a highly important process for the tight control of gene expression. Inherently unstable mRNAs contain AU-rich elements (AREs) in the 3' untranslated regions that direct rapid mRNA decay by interaction with decay-promoting ARE-binding proteins (ARE-BPs). The decay of ARE-containing mRNAs is regulated by signaling pathways that are believed to directly target ARE-BPs. Here, we show that ... [more]

RNA May. 01, 2008; 14(5);950-9 [Pubmed: 18326031]

Throughput

  • Low Throughput

Additional Notes

  • Figure 7

Curated By

  • BioGRID