BAIT
MAPK3
ERK1, ERT2, Erk-1, Esrk1, MAPK1, MNK1, Prkm3, p44, p44erk1, p44mapk
mitogen activated protein kinase 3
GO Process (37)
GO Function (7)
GO Component (15)
Gene Ontology Biological Process
- BMP signaling pathway [ISO]
- DNA damage induced protein phosphorylation [ISO]
- MAPK cascade [IMP, ISO]
- MAPK import into nucleus [IDA]
- arachidonic acid metabolic process [IEP]
- cartilage development [ISO]
- caveolin-mediated endocytosis [TAS]
- cellular response to DNA damage stimulus [ISO]
- cellular response to mechanical stimulus [ISO]
- interleukin-1-mediated signaling pathway [ISO]
- intracellular signal transduction [IDA, TAS]
- lipopolysaccharide-mediated signaling pathway [ISO]
- negative regulation of apolipoprotein binding [ISO]
- organ morphogenesis [ISO]
- peptidyl-serine phosphorylation [IMP]
- peptidyl-tyrosine autophosphorylation [ISO]
- phosphorylation [ISO]
- positive regulation of ERK1 and ERK2 cascade [ISO]
- positive regulation of histone acetylation [ISO]
- positive regulation of histone phosphorylation [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription, DNA-templated [IEP]
- positive regulation of translation [IMP]
- protein complex assembly [IMP]
- protein phosphorylation [IDA, ISO, TAS]
- regulation of Golgi inheritance [TAS]
- regulation of cytoskeleton organization [TAS]
- regulation of early endosome to late endosome transport [TAS]
- regulation of sequence-specific DNA binding transcription factor activity [ISO]
- regulation of stress-activated MAPK cascade [TAS]
- response to epidermal growth factor [ISO, ISS]
- response to exogenous dsRNA [ISO]
- response to lipopolysaccharide [ISO]
- response to toxic substance [IDA]
- sensory perception of pain [ISO]
- transcription, DNA-templated [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [TAS]
- caveola [TAS]
- cytoplasm [ISO]
- cytoskeleton [TAS]
- cytosol [IDA, ISO, TAS]
- early endosome [TAS]
- extracellular vesicular exosome [ISO]
- focal adhesion [TAS]
- late endosome [TAS]
- microtubule cytoskeleton [ISO]
- mitochondrion [TAS]
- nucleoplasm [IDA, ISO]
- nucleus [IDA, ISO, TAS]
- protein complex [IDA]
- pseudopodium [ISO]
Rattus norvegicus
PREY
PTGS2
COX-2, Cox2
prostaglandin-endoperoxide synthase 2
GO Process (57)
GO Function (7)
GO Component (5)
Gene Ontology Biological Process
- angiogenesis [IMP]
- bone mineralization [IMP]
- brown fat cell differentiation [ISO]
- cellular response to ATP [IEP]
- cellular response to UV [IEP]
- cellular response to hypoxia [ISO]
- cellular response to mechanical stimulus [IEP]
- cyclooxygenase pathway [ISO, ISS]
- decidualization [IMP, ISO]
- embryo implantation [IMP, ISO]
- hair cycle [IEP]
- inflammatory response [IMP]
- learning [IMP]
- maintenance of blood-brain barrier [IMP]
- memory [IMP]
- negative regulation of calcium ion transport [IMP]
- negative regulation of cell cycle [IGI]
- negative regulation of cell proliferation [IDA]
- negative regulation of smooth muscle contraction [IMP]
- negative regulation of synaptic transmission, dopaminergic [IMP]
- ovulation [IMP]
- positive regulation of NF-kappaB import into nucleus [IMP]
- positive regulation of apoptotic process [IMP]
- positive regulation of brown fat cell differentiation [ISO]
- positive regulation of cell death [IMP]
- positive regulation of cell migration involved in sprouting angiogenesis [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of fever generation [IDA, ISO]
- positive regulation of fibroblast growth factor production [IMP]
- positive regulation of nitric oxide biosynthetic process [IMP]
- positive regulation of platelet-derived growth factor production [IMP]
- positive regulation of smooth muscle cell proliferation [IMP]
- positive regulation of smooth muscle contraction [IMP]
- positive regulation of synaptic plasticity [IMP]
- positive regulation of synaptic transmission, glutamatergic [IMP]
- positive regulation of transforming growth factor beta production [IMP]
- positive regulation of vasoconstriction [IMP]
- positive regulation vascular endothelial growth factor production [IMP]
- prostaglandin biosynthetic process [IMP, ISO]
- regulation of blood pressure [ISO, ISS]
- regulation of cell proliferation [ISO]
- response to cytokine [IMP]
- response to drug [IEP]
- response to estradiol [IEP]
- response to fatty acid [IEP]
- response to fructose [IEP]
- response to glucocorticoid [IEP]
- response to lipopolysaccharide [IMP]
- response to lithium ion [IEP]
- response to manganese ion [IEP]
- response to organic cyclic compound [IEP]
- response to organic substance [IEP]
- response to organonitrogen compound [IEP]
- response to radiation [IEP]
- response to tumor necrosis factor [IEP]
- response to vitamin D [IEP]
- sensory perception of pain [IMP]
Gene Ontology Molecular Function- enzyme binding [ISO]
- heme binding [ISO, ISS]
- lipid binding [IMP]
- oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen [IDA]
- prostaglandin-endoperoxide synthase activity [ISO, ISS]
- protein binding [IPI]
- protein homodimerization activity [ISO]
- enzyme binding [ISO]
- heme binding [ISO, ISS]
- lipid binding [IMP]
- oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen [IDA]
- prostaglandin-endoperoxide synthase activity [ISO, ISS]
- protein binding [IPI]
- protein homodimerization activity [ISO]
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Resveratrol is pro-apoptotic and thyroid hormone is anti-apoptotic in glioma cells: both actions are integrin and ERK mediated.
The stilbene resveratrol (RV) initiates p53-dependent apoptosis via plasma membrane integrin alphaVbeta3 in human cancer cells. A thyroid hormone (L-thyroxine, T(4)) membrane receptor also exists on alphaVbeta3. Stilbene and T(4) signals are both transduced by extracellular-regulated kinases 1 and 2 (ERK1/2); however, T(4) promotes cell proliferation in cancer cells, whereas RV is pro-apoptotic. Thyroid hormone has been shown to interfere ... [more]
Carcinogenesis Jan. 01, 2008; 29(1);62-9 [Pubmed: 17984113]
Throughput
- Low Throughput
Additional Notes
- Figure 4
Curated By
- BioGRID