SPOP
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
DDIT3
Gene Ontology Biological Process
- activation of signaling protein activity involved in unfolded protein response [TAS]
- cell redox homeostasis [IDA]
- cellular protein metabolic process [TAS]
- cellular response to DNA damage stimulus [TAS]
- endoplasmic reticulum unfolded protein response [TAS]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [IDA, IMP, TAS]
- mRNA transcription from RNA polymerase II promoter [IDA]
- negative regulation of canonical Wnt signaling pathway [ISS]
- negative regulation of determination of dorsal identity [IDA]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of interleukin-8 production [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress [ISS]
- positive regulation of transcription, DNA-templated [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- regulation of DNA-templated transcription in response to stress [TAS]
- regulation of transcription involved in anterior/posterior axis specification [ISS]
- regulation of transcription, DNA-templated [IMP]
- response to endoplasmic reticulum stress [IDA]
- response to unfolded protein [IDA]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- protein binding [IPI]
- protein heterodimerization activity [TAS]
- sequence-specific DNA binding transcription factor activity [NAS]
- transcription corepressor activity [TAS]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [ISS]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- protein binding [IPI]
- protein heterodimerization activity [TAS]
- sequence-specific DNA binding transcription factor activity [NAS]
- transcription corepressor activity [TAS]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [ISS]
Gene Ontology Cellular Component
- CHOP-C/EBP complex [TAS]
- cytosol [TAS]
- nucleoplasm [IDA, TAS]
- nucleus [IDA, TAS]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Destruction of DDIT3/CHOP protein by wild-type SPOP but not prostate cancer-associated mutants.
Characterization of the exome and genome of prostate cancers by next-generation sequencing has identified numerous genetic alternations. SPOP (speckle-type POZ protein) was identified as one of the most frequently affected genes by somatic point mutations in prostate cancer, suggesting SPOP is potentially a key driver for prostate cancer development and progression. However, how SPOP mutations contribute to prostate cancer remains ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DDIT3 SPOP | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| DDIT3 SPOP | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID