Publication

Systematic Genetic Screens Reveal the Dynamic Global Functional Organization of the Bacterial Translation Machinery.

Gagarinova A, Stewart G, Samanfar B, Phanse S, White CA, Aoki H, Deineko V, Beloglazova N, Yakunin AF, Golshani A, Brown ED, Babu M, Emili A

Bacterial protein synthesis is an essential, conserved, and environmentally responsive process. Yet, many of its components and dependencies remain unidentified. To address this gap, we used quantitative synthetic genetic arrays to map functional relationships among >48,000 gene pairs in Escherichia coli under four culture conditions differing in temperature and nutrient availability. The resulting data provide global functional insights into the roles and ... [more]

Quantitative Score

• 0.163464297 [Confidence Score]

Throughput

• High Throughput

Additional Notes

• Conditions: Rich Media 32Deg C
• Genetic interactions were considered significant if they had an Epsilon score >= 0.153831326 for positive interactions (alleviating interactions) and Epsilon score <= -0.099945541 for negative interactions (aggravating interactions).

Curated By

• BioGRID