BAIT

TFB1

TFIIH/NER complex subunit TFB1, L000002284, YDR311W

Subunit of TFIIH and nucleotide excision repair factor 3 complexes; required for nucleotide excision repair, target for transcriptional activators; relocalizes to the cytosol in response to hypoxia

GO Process (4)

GO Function (3)

GO Component (5)

Gene Ontology Biological Process

nucleotide-excision repair [IMP]

phosphorylation of RNA polymerase II C-terminal domain [IDA]

transcription from RNA polymerase I promoter [IMP]

transcription from RNA polymerase II promoter [IDA]

Gene Ontology Molecular Function

core RNA polymerase binding transcription factor activity [IC]
phosphatidylinositol-3-phosphate binding [IDA]
phosphatidylinositol-5-phosphate binding [IDA]

Gene Ontology Cellular Component

core TFIIH complex [IDA]

cytosol [IDA]

holo TFIIH complex [IDA]

nucleotide-excision repair factor 3 complex [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

TFB2

TFIIH/NER complex subunit TFB2, L000003495, YPL122C

Subunit of TFIIH and nucleotide excision repair factor 3 complexes; involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH

GO Process (3)

GO Function (2)

GO Component (3)

Gene Ontology Biological Process

nucleotide-excision repair [IMP]

phosphorylation of RNA polymerase II C-terminal domain [IDA]

transcription from RNA polymerase II promoter [IDA]

Gene Ontology Molecular Function

core RNA polymerase binding transcription factor activity [IC]
double-stranded DNA binding [IDA]

Gene Ontology Cellular Component

core TFIIH complex [IDA]

holo TFIIH complex [IDA]

nucleotide-excision repair factor 3 complex [IPI]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

Revised subunit structure of yeast transcription factor IIH (TFIIH) and reconciliation with human TFIIH.

Takagi Y, Komori H, Chang WH, Hudmon A, Erdjument-Bromage H, Tempst P, Kornberg RD

Tfb4 is identified as a subunit of the core complex of yeast RNA polymerase II general transcription factor IIH (TFIIH) by affinity purification, by peptide sequence analysis, and by expression of the entire complex in insect cells. Tfb3, previously identified as a component of the core complex, is shown instead to form a complex with cdk and cyclin subunits of ... [more]

J. Biol. Chem. Nov. 07, 2003; 278(45);43897-900 [Pubmed: 14500720]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TFB1 TFB2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	10	BioGRID	3612483
TFB1 TFB2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Takagi Y (2005)	Low	-	BioGRID	-
TFB2 TFB1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.3405	BioGRID	1955194

Curated By

BioGRID

Interaction Summary

TFB1

Gene Ontology Biological Process

Gene Ontology Molecular Function

core RNA polymerase binding transcription factor activity [IC]phosphatidylinositol-3-phosphate binding [IDA]phosphatidylinositol-5-phosphate binding [IDA]

Gene Ontology Cellular Component

TFB2

Gene Ontology Biological Process

Gene Ontology Molecular Function

core RNA polymerase binding transcription factor activity [IC]double-stranded DNA binding [IDA]

Gene Ontology Cellular Component

Co-purification

Publication

Revised subunit structure of yeast transcription factor IIH (TFIIH) and reconciliation with human TFIIH.

Throughput

Related interactions

Curated By

core RNA polymerase binding transcription factor activity [IC]
phosphatidylinositol-3-phosphate binding [IDA]
phosphatidylinositol-5-phosphate binding [IDA]

core RNA polymerase binding transcription factor activity [IC]
double-stranded DNA binding [IDA]