BAIT

TFB1

TFIIH/NER complex subunit TFB1, L000002284, YDR311W

Subunit of TFIIH and nucleotide excision repair factor 3 complexes; required for nucleotide excision repair, target for transcriptional activators; relocalizes to the cytosol in response to hypoxia

GO Process (4)

GO Function (3)

GO Component (5)

Gene Ontology Biological Process

nucleotide-excision repair [IMP]

phosphorylation of RNA polymerase II C-terminal domain [IDA]

transcription from RNA polymerase I promoter [IMP]

transcription from RNA polymerase II promoter [IDA]

Gene Ontology Molecular Function

core RNA polymerase binding transcription factor activity [IC]
phosphatidylinositol-3-phosphate binding [IDA]
phosphatidylinositol-5-phosphate binding [IDA]

Gene Ontology Cellular Component

core TFIIH complex [IDA]

cytosol [IDA]

holo TFIIH complex [IDA]

nucleotide-excision repair factor 3 complex [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SSL1

TFIIH/NER complex subunit SSL1, L000002085, YLR005W

Subunit of the core form of RNA polymerase transcription factor TFIIH; has both protein kinase and DNA-dependent ATPase/helicase activities; essential for transcription and nucleotide excision repair; interacts with Tfb4p

UPS Project

GO Process (3)

GO Function (1)

GO Component (3)

Gene Ontology Biological Process

nucleotide-excision repair [IMP]

phosphorylation of RNA polymerase II C-terminal domain [IDA]

transcription from RNA polymerase II promoter [IDA]

Gene Ontology Molecular Function

core RNA polymerase binding transcription factor activity [IC]

Gene Ontology Cellular Component

core TFIIH complex [IDA]

holo TFIIH complex [IDA]

nucleotide-excision repair factor 3 complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

Revised subunit structure of yeast transcription factor IIH (TFIIH) and reconciliation with human TFIIH.

Takagi Y, Komori H, Chang WH, Hudmon A, Erdjument-Bromage H, Tempst P, Kornberg RD

Tfb4 is identified as a subunit of the core complex of yeast RNA polymerase II general transcription factor IIH (TFIIH) by affinity purification, by peptide sequence analysis, and by expression of the entire complex in insect cells. Tfb3, previously identified as a component of the core complex, is shown instead to form a complex with cdk and cyclin subunits of ... [more]

J. Biol. Chem. Nov. 07, 2003; 278(45);43897-900 [Pubmed: 14500720]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SSL1 TFB1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2002)	High	-	BioGRID	-
TFB1 SSL1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2002)	High	-	BioGRID	-
TFB1 SSL1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
SSL1 TFB1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
TFB1 SSL1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2006)	High	-	BioGRID	-
TFB1 SSL1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	10	BioGRID	3612486
TFB1 SSL1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Takagi Y (2005)	Low	-	BioGRID	-
SSL1 TFB1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Warfield L (2016)	Low	-	BioGRID	-
TFB1 SSL1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.1357	BioGRID	1927219
SSL1 TFB1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.3053	BioGRID	1942746
SSL1 TFB1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Bardwell L (1994)	Low	-	BioGRID	-
TFB1 SSL1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Luo J (2015)	Low	-	BioGRID	2346028

Curated By

BioGRID

Interaction Summary

TFB1

Gene Ontology Biological Process

Gene Ontology Molecular Function

core RNA polymerase binding transcription factor activity [IC]phosphatidylinositol-3-phosphate binding [IDA]phosphatidylinositol-5-phosphate binding [IDA]

Gene Ontology Cellular Component

SSL1

Gene Ontology Biological Process

Gene Ontology Molecular Function

core RNA polymerase binding transcription factor activity [IC]

Gene Ontology Cellular Component

Co-purification

Publication

Revised subunit structure of yeast transcription factor IIH (TFIIH) and reconciliation with human TFIIH.

Throughput

Related interactions

Curated By

core RNA polymerase binding transcription factor activity [IC]
phosphatidylinositol-3-phosphate binding [IDA]
phosphatidylinositol-5-phosphate binding [IDA]