Biochemical Activity (Ubiquitination)

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Identification of substrates of SMURF1 ubiquitin ligase activity utilizing protein microarrays.

Andrews PS, Schneider S, Yang E, Michaels M, Chen H, Tang J, Emkey R

The ubiquitin proteasome pathway (UPP) has been implicated in a number of pathogenic diseases: cancer, inflammation, metabolic disorders, and viral infection. The human genome contains well over 500 genes encoding proteins involved in the UPP. Ubiquitin ligases (E3s) comprise the largest subset of these genes, and together with an E2 partner, provide the substrate selectivity required for regulating cellular proteins ... [more]

Assay Drug Dev Technol Aug. 01, 2010; 8(4);471-87 [Pubmed: 20804422]

Throughput

  • High Throughput

Additional Notes

  • peptide in vitro array

Curated By

  • BioGRID