An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Two distinct classes of CCAAT box elements that bind nuclear factor-Y/alpha-actinin-4: potential role in human CYP1A1 regulation.

Poch MT, Al-Kassim L, Smolinski SM, Hines RN

A negative regulatory element (NRE1; position -794 to -774) was previously identified that mediates the downregulation of CYP1A1, including partial suppression of Ah receptor-dependent induction. The CCAAT-box binding protein, nuclear factor-Y (NF-Y), is a component of one of two protein complexes that specifically and competitively bind the CYP1A1 NRE1 in vitro with nearly equal affinity. The second complex involves an ... [more]

Toxicol. Appl. Pharmacol. Sep. 15, 2004; 199(3);239-50 [Pubmed: 15364540]

Throughput

Low Throughput

Additional Notes

Figure 7

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ACTN4 NFYA	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Poch MT (2004)	Low	-	BioGRID	1534106

Curated By

BioGRID

Interaction Summary

NFYA

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [IDA]core promoter sequence-specific DNA binding [ISS]protein binding [IPI]transcription regulatory region DNA binding [IDA]

Gene Ontology Cellular Component

ACTN4

Gene Ontology Biological Process

Gene Ontology Molecular Function

actin binding [TAS]actin filament binding [IDA]integrin binding [TAS]nucleoside binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]protein homodimerization activity [TAS]