An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling.

Zhang F, Yao J, Ke J, Zhang L, Lam VQ, Xin XF, Zhou XE, Chen J, Brunzelle J, Griffin PR, Zhou M, Xu HE, Melcher K, He SY

The plant hormone jasmonate plays crucial roles in regulating plant responses to herbivorous insects and microbial pathogens and is an important regulator of plant growth and development. Key mediators of jasmonate signalling include MYC transcription factors, which are repressed by jasmonate ZIM-domain (JAZ) transcriptional repressors in the resting state. In the presence of active jasmonate, JAZ proteins function as jasmonate ... [more]

Nature Sep. 10, 2015; 525(7568);269-73 [Pubmed: 26258305]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AT5G46760 TIFY7	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Zhang F (2015)	Low	-	BioGRID	-
TIFY7 AT5G46760	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Fernandez-Calvo P (2011)	Low	-	BioGRID	-
TIFY7 AT5G46760	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Niu Y (2011)	Low	-	BioGRID	-
AT5G46760 TIFY7	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Fernandez-Calvo P (2011)	Low	-	BioGRID	-
TIFY7 AT5G46760	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Fernandez-Calvo P (2011)	Low	-	BioGRID	-
TIFY7 AT5G46760	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Niu Y (2011)	Low	-	BioGRID	-
TIFY7 AT5G46760	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Zhang F (2015)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

AT5G46760

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [ISS]bHLH transcription factor binding [IPI]protein binding [IPI]sequence-specific DNA binding transcription factor activity [ISS]

Gene Ontology Cellular Component

TIFY7