BAIT

WRKY18

ARABIDOPSIS THALIANA WRKY DNA-BINDING PROTEIN 18, ATWRKY18, F11C18.16, WRKY DNA-binding protein 18, AT4G31800
WRKY DNA-binding protein 18
Arabidopsis thaliana (Columbia)
PREY

WRKY40

ATWRKY40, F23A5.19, F23A5_19, WRKY DNA-binding protein 40, AT1G80840
putative WRKY transcription factor 40
Arabidopsis thaliana (Columbia)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Regulation of oncogene expression in T-DNA-transformed host plant cells.

Zhang Y, Lee CW, Wehner N, Imdahl F, Svetlana V, Weiste C, Droege-Laser W, Deeken R

Virulent Agrobacterium tumefaciens strains integrate their T-DNA into the plant genome where the encoded agrobacterial oncogenes are expressed and cause crown gall disease. Essential for crown gall development are IaaH (indole-3-acetamide hydrolase), IaaM (tryptophan monooxygenase) and Ipt (isopentenyl transferase), which encode enzymes for the biosynthesis of auxin (IaaH, IaaM) and cytokinin (Ipt). Although these oncogenes are well studied as the ... [more]

PLoS Pathog. Jan. 01, 2015; 11(1);e1004620 [Pubmed: 25615824]

Throughput

  • Low Throughput

Additional Notes

  • triple wrky18/60/40 mutant plants develops smaller crown galls than wild-type

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
WRKY18 WRKY40
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
WRKY18 WRKY40
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-
WRKY40 WRKY18
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID