BAIT
ITGA2
CD49B, DX5
integrin alpha 2
GO Process (27)
GO Function (7)
GO Component (11)
Gene Ontology Biological Process
- cell adhesion mediated by integrin [ISO]
- cell-matrix adhesion [ISO]
- cell-substrate adhesion [ISO]
- collagen-activated signaling pathway [ISO]
- detection of mechanical stimulus involved in sensory perception of pain [ISO]
- establishment of protein localization [ISO]
- focal adhesion assembly [ISO]
- hypotonic response [ISO]
- integrin-mediated signaling pathway [ISO]
- positive regulation of DNA binding [ISO]
- positive regulation of alkaline phosphatase activity [ISO]
- positive regulation of cell adhesion [ISO]
- positive regulation of cell projection organization [ISO]
- positive regulation of collagen binding [ISO]
- positive regulation of collagen biosynthetic process [ISO]
- positive regulation of epithelial cell migration [ISO]
- positive regulation of inflammatory response [ISO]
- positive regulation of leukocyte migration [ISO]
- positive regulation of phagocytosis, engulfment [ISO]
- positive regulation of positive chemotaxis [ISO]
- positive regulation of smooth muscle cell migration [ISO]
- positive regulation of smooth muscle cell proliferation [ISO]
- positive regulation of smooth muscle contraction [ISO]
- positive regulation of translation [ISO]
- positive regulation of transmission of nerve impulse [ISO]
- skin morphogenesis [ISO]
- substrate-dependent cell migration [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
LYN
AA407514, Hck-2, p53Lyn, p56Lyn, RP23-49A12.2
Yamaguchi sarcoma viral (v-yes-1) oncogene homolog
GO Process (66)
GO Function (16)
GO Component (17)
Gene Ontology Biological Process
- B cell homeostasis [IMP]
- B cell receptor signaling pathway [IDA]
- Fc receptor mediated inhibitory signaling pathway [IMP]
- Fc receptor mediated stimulatory signaling pathway [IMP]
- cellular response to DNA damage stimulus [IBA, ISO]
- cellular response to peptide hormone stimulus [IBA]
- cellular response to retinoic acid [ISO]
- central nervous system development [IBA]
- chemotaxis [IBA]
- cytokine secretion [ISO]
- dendritic cell differentiation [IMP]
- erythrocyte differentiation [IMP]
- hemoglobin biosynthetic process [TAS]
- hemopoiesis [IMP]
- histamine secretion by mast cell [ISO]
- immune response-regulating cell surface receptor signaling pathway [IMP]
- intracellular signal transduction [IDA]
- lipopolysaccharide-mediated signaling pathway [IMP]
- negative regulation of B cell proliferation [IMP]
- negative regulation of ERK1 and ERK2 cascade [IMP]
- negative regulation of MAP kinase activity [IMP]
- negative regulation of cell proliferation [IMP, ISO]
- negative regulation of intracellular signal transduction [IMP]
- negative regulation of mast cell proliferation [IMP]
- negative regulation of myeloid leukocyte differentiation [IMP]
- negative regulation of protein phosphorylation [IMP]
- negative regulation of toll-like receptor 2 signaling pathway [IMP]
- negative regulation of toll-like receptor 4 signaling pathway [IMP]
- neuron projection development [IMP]
- peptidyl-tyrosine autophosphorylation [IBA]
- peptidyl-tyrosine phosphorylation [IDA, ISO]
- platelet degranulation [IMP]
- positive regulation of B cell receptor signaling pathway [IGI]
- positive regulation of Fc receptor mediated stimulatory signaling pathway [ISO]
- positive regulation of cell migration [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of cellular component movement [ISO]
- positive regulation of dendritic cell apoptotic process [IMP]
- positive regulation of glial cell proliferation [ISO]
- positive regulation of mast cell proliferation [ISO]
- positive regulation of neuron projection development [ISO]
- positive regulation of oligodendrocyte progenitor proliferation [ISO]
- positive regulation of peptidyl-tyrosine phosphorylation [IDA]
- positive regulation of phosphatidylinositol 3-kinase activity [IMP]
- positive regulation of phosphorylation [ISO]
- positive regulation of stress-activated protein kinase signaling cascade [ISO]
- positive regulation of tyrosine phosphorylation of STAT protein [IMP]
- protein autophosphorylation [IDA, ISO, TAS]
- protein phosphorylation [ISO]
- regulation of B cell apoptotic process [IMP]
- regulation of B cell receptor signaling pathway [IMP]
- regulation of ERK1 and ERK2 cascade [IMP]
- regulation of cell adhesion mediated by integrin [ISO]
- regulation of cytokine production [IMP]
- regulation of cytokine secretion [IMP]
- regulation of erythrocyte differentiation [IMP]
- regulation of inflammatory response [IMP]
- regulation of mast cell activation [IMP]
- regulation of mast cell degranulation [IMP]
- regulation of monocyte chemotaxis [ISO]
- regulation of platelet aggregation [IMP]
- regulation of release of sequestered calcium ion into cytosol [IGI, ISO]
- response to hormone [IDA]
- signal transduction [TAS]
- tolerance induction to self antigen [IMP]
- transmembrane receptor protein tyrosine kinase signaling pathway [IBA, ISO]
Gene Ontology Molecular Function- SH3 domain binding [IPI]
- enzyme binding [ISO]
- erythropoietin receptor binding [TAS]
- gamma-tubulin binding [ISO]
- glycosphingolipid binding [ISO]
- integrin binding [ISO]
- ion channel binding [ISO]
- non-membrane spanning protein tyrosine kinase activity [IBA]
- phosphoprotein binding [ISO]
- platelet-derived growth factor receptor binding [ISO]
- protein binding [IPI]
- protein complex binding [IPI, ISO]
- protein kinase activity [IDA]
- protein tyrosine kinase activity [IDA, ISO]
- receptor binding [IBA, ISO]
- ubiquitin protein ligase binding [ISO]
- SH3 domain binding [IPI]
- enzyme binding [ISO]
- erythropoietin receptor binding [TAS]
- gamma-tubulin binding [ISO]
- glycosphingolipid binding [ISO]
- integrin binding [ISO]
- ion channel binding [ISO]
- non-membrane spanning protein tyrosine kinase activity [IBA]
- phosphoprotein binding [ISO]
- platelet-derived growth factor receptor binding [ISO]
- protein binding [IPI]
- protein complex binding [IPI, ISO]
- protein kinase activity [IDA]
- protein tyrosine kinase activity [IDA, ISO]
- receptor binding [IBA, ISO]
- ubiquitin protein ligase binding [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- cell [IMP]
- cell-cell adherens junction [ISO]
- cytoplasm [IDA]
- extracellular vesicular exosome [ISO]
- extrinsic component of cytoplasmic side of plasma membrane [IBA]
- integrin alpha2-beta1 complex [ISO]
- mast cell granule [ISO]
- membrane [ISO]
- membrane raft [ISO]
- mitochondrial crista [ISO]
- mitochondrial intermembrane space [ISO]
- mitochondrial membrane [ISO]
- nucleus [IDA, ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [IDA, ISO]
- postsynaptic density [ISO]
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Rhodocytin induces platelet aggregation by interacting with glycoprotein Ia/IIa (GPIa/IIa, Integrin alpha 2beta 1). Involvement of GPIa/IIa-associated src and protein tyrosine phosphorylation.
Although glycoprotein Ia/IIa (GPIa/IIa, integrin alpha(2)beta(1)) has established its role as a collagen receptor, it remains unclear whether GPIa/IIa mediates activation signals. In this study, we show that rhodocytin, purified from the Calloselasma rhodostoma venom, induces platelet aggregation, which can be blocked by anti-GPIa monoclonal antibodies. Studies with rhodocytin-coupled beads and liposomes loaded with recombinant GPIa/IIa demonstrated that rhodocytin directly ... [more]
J. Biol. Chem. Jan. 12, 2001; 276(2);1643-52 [Pubmed: 11038351]
Throughput
- Low Throughput
Curated By
- BioGRID