UGT2B7
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CYP3A4
Gene Ontology Biological Process
- alkaloid catabolic process [IDA]
- androgen metabolic process [TAS]
- calcitriol biosynthetic process from calciol [IDA]
- drug catabolic process [IDA, IMP]
- drug metabolic process [IDA]
- exogenous drug catabolic process [IDA]
- heterocycle metabolic process [IDA]
- lipid metabolic process [TAS]
- monoterpenoid metabolic process [IDA]
- oxidation-reduction process [IDA]
- oxidative demethylation [IDA]
- small molecule metabolic process [TAS]
- steroid catabolic process [IMP]
- steroid metabolic process [IMP]
- vitamin D metabolic process [IC]
- xenobiotic metabolic process [TAS]
Gene Ontology Molecular Function- caffeine oxidase activity [IDA]
- enzyme binding [IPI]
- iron ion binding [IDA]
- monooxygenase activity [IDA, ISS]
- oxidoreductase activity [IDA]
- oxygen binding [TAS]
- steroid binding [IDA]
- steroid hydroxylase activity [IMP]
- testosterone 6-beta-hydroxylase activity [IMP]
- vitamin D 24-hydroxylase activity [IDA]
- vitamin D3 25-hydroxylase activity [IDA]
- caffeine oxidase activity [IDA]
- enzyme binding [IPI]
- iron ion binding [IDA]
- monooxygenase activity [IDA, ISS]
- oxidoreductase activity [IDA]
- oxygen binding [TAS]
- steroid binding [IDA]
- steroid hydroxylase activity [IMP]
- testosterone 6-beta-hydroxylase activity [IMP]
- vitamin D 24-hydroxylase activity [IDA]
- vitamin D3 25-hydroxylase activity [IDA]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Coimmunoprecipitation of UDP-glucuronosyltransferase isoforms and cytochrome P450 3A4.
Coimmunoprecipitation was used to investigate protein-protein interactions between several UDP-glucuronosyltransferase (UGT) isoforms and cytochrome P450 3A4. Solubilized human liver microsomes were incubated with specific antibodies to UGT2B7, UGT1A6, UGT1A1, and CYP3A4, and the immunoprecipitates were run on SDS-polyacrylamide gel electrophoresis. Western blots showed that UGT2B7, UGT1A6, UGT1A1, and CYP3A4 were successfully immunoprecipitated with the specific antibodies for each enzyme. Upon ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CYP3A4 UGT2B7 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| UGT2B7 CYP3A4 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 1526526 | |
| CYP3A4 UGT2B7 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 1528159 | |
| UGT2B7 CYP3A4 | Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe. | Low | - | BioGRID | 1526527 |
Curated By
- BioGRID