BAIT

AIM1

YAL046C

Protein involved in mitochondrial function or organization; null mutant displays elevated frequency of mitochondrial genome loss

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

NFU1

NUB1, L000004771, YKL040C

Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria

GO Process (3)

GO Function (0)

GO Component (2)

Gene Ontology Biological Process

cellular iron ion homeostasis [IMP, ISS]

intracellular sequestering of iron ion [IMP]

iron-sulfur cluster assembly [IMP]

Gene Ontology Cellular Component

mitochondrial matrix [IDA]

mitochondrion [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Role of Nfu1 and Bol3 in iron-sulfur cluster transfer to mitochondrial clients.

Melber A, Na U, Vashisht A, Weiler BD, Lill R, Wohlschlegel JA, Winge DR

Iron-sulfur (Fe-S) clusters are essential for many cellular processes, ranging from aerobic respiration, metabolite biosynthesis, ribosome assembly and DNA repair. Mutations in NFU1 and BOLA3 have been linked to genetic diseases with defects in mitochondrial Fe-S centers. Through genetic studies in yeast, we demonstrate that Nfu1 functions in a late step of [4Fe-4S] cluster biogenesis that is of heightened importance ... [more]

Elife Aug. 17, 2016; 5(0); [Pubmed: 27532773]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AIM1 NFU1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.2206	BioGRID	2076236
NFU1 AIM1	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Melber A (2016)	Low	-	BioGRID	1537714

Curated By

BioGRID

Interaction Summary