BAIT
CDC34
DNA6, UBC3, SCF E2 ubiquitin-protein ligase catalytic subunit CDC34, L000000271, YDR054C
Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress
GO Process (6)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [TAS]
- G2/M transition of mitotic cell cycle [IGI]
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [IDA]
- protein autoubiquitination [IDA, IMP]
- protein polyubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
GCN4
AAS101, AAS3, ARG9, amino acid starvation-responsive transcription factor GCN4, L000000683, YEL009C
bZIP transcriptional activator of amino acid biosynthetic genes; activator responds to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
GO Process (5)
GO Function (7)
GO Component (1)
Gene Ontology Biological Process
- negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- nitrogen catabolite activation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of RNA polymerase II transcriptional preinitiation complex assembly [IDA, IGI, IMP]
- positive regulation of transcription initiation from RNA polymerase II promoter [IGI, IMP]
Gene Ontology Molecular Function- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [IPI]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA, IMP, IPI]
- RNA polymerase II transcription factor recruiting transcription factor activity [IDA, IMP, IPI]
- TFIID-class binding transcription factor activity [IPI]
- chromatin binding [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, IMP]
- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [IPI]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA, IMP, IPI]
- RNA polymerase II transcription factor recruiting transcription factor activity [IDA, IMP, IPI]
- TFIID-class binding transcription factor activity [IPI]
- chromatin binding [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, IMP]
Saccharomyces cerevisiae (S288c)
Dosage Lethality
A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.
Publication
Degradation of the transcription factor Gcn4 requires the kinase Pho85 and the SCF(CDC4) ubiquitin-ligase complex.
Gcn4, a yeast transcriptional activator that promotes the expression of amino acid and purine biosynthesis genes, is rapidly degraded in rich medium. Here we report that SCF(CDC4), a recently characterized protein complex that acts in conjunction with the ubiquitin-conjugating enzyme Cdc34 to degrade cell cycle regulators, is also necessary for the degradation of the transcription factor Gcn4. Degradation of Gcn4 ... [more]
Mol. Biol. Cell Mar. 01, 2000; 11(3);915-27 [Pubmed: 10712509]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID