BAIT

PMS1

L000001457, YNL082W
ATP-binding protein required for mismatch repair; required for both mitosis and meiosis; functions as a heterodimer with Mlh1p; binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL
Saccharomyces cerevisiae (S288c)
PREY

RAD30

DBH1, L000003523, YDR419W
DNA polymerase eta; involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; involved in formation of post-replicative damage-induced genome-wide cohesion; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV
GO Process (4)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Lethality

A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.

Publication

Mutator effects of overproducing DNA polymerase eta (Rad30) and its catalytically inactive variant in yeast.

Pavlov YI, Nguyen D, Kunkel TA

DNA polymerase eta synthesizes DNA in vitro with low fidelity. Based on this, here we report the effects of deletion or increased expression of yeast RAD30 gene, encoding for polymerase eta (Pol eta), on spontaneous mutagenesis in vivo. Deletion of RAD30 did not affect spontaneous mutagenesis. Overproduction of Rad30p was slightly mutagenic in a wild-type yeast strain and moderately mutagenic ... [more]

Mutat. Res. Jul. 01, 2001; 478(1);129-39 [Pubmed: 11406177]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)
  • phenotype: mutation frequency (APO:0000198)

Curated By

  • BioGRID