BAIT

BEM2

IPL2, SUP9, TSL1, L000000168, YER155C
Rho GTPase activating protein (RhoGAP); involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence; potential GAP for Rho4p
Saccharomyces cerevisiae (S288c)
PREY

RHO1

Rho family GTPase RHO1, L000001630, YPR165W
GTP-binding protein of the rho subfamily of Ras-like proteins; involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Control of cellular morphogenesis by the Ip12/Bem2 GTPase-activating protein: possible role of protein phosphorylation.

Kim YJ, Francisco L, Chen GC, Marcotte E, Chan CS

The IPL2 gene is known to be required for normal polarized cell growth in the budding yeast Saccharomyces cerevisiae. We now show that IPL2 is identical to the previously identified BEM2 gene. bem2 mutants are defective in bud site selection at 26 degrees C and localized cell surface growth and organization of the actin cytoskeleton at 37 degrees C. BEM2 ... [more]

J. Cell Biol. Dec. 01, 1994; 127(5);1381-94 [Pubmed: 7962097]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: heat sensitivity (APO:0000147)
  • phenotype: budding pattern (APO:0000200)

Additional Notes

  • in strain overexpression both RHO1 and RHO2

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BEM2 RHO1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
157647

Curated By

  • BioGRID