BAIT

TAF3

TAF47, TafII47, L000004154, YPL011C

TFIID subunit (47 kDa); involved in promoter binding and RNA polymerase II transcription initiation

GO Process (2)

GO Function (2)

GO Component (1)

Gene Ontology Biological Process

RNA polymerase II transcriptional preinitiation complex assembly [IC]

transcription from RNA polymerase II promoter [IDA]

Gene Ontology Molecular Function

RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]
chromatin binding [IDA]

Gene Ontology Cellular Component

transcription factor TFIID complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

TAF13

FUN81, TAF19, TafII19, L000000650, YML098W

TFIID subunit (19 kDa); involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors

GO Process (2)

GO Function (1)

GO Component (2)

Gene Ontology Biological Process

RNA polymerase II transcriptional preinitiation complex assembly [IMP]

transcription from RNA polymerase II promoter [IDA]

Gene Ontology Molecular Function

RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]

Gene Ontology Cellular Component

nucleus [IDA]

transcription factor TFIID complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Histone folds mediate selective heterodimerization of yeast TAF(II)25 with TFIID components yTAF(II)47 and yTAF(II)65 and with SAGA component ySPT7.

Gangloff YG, Sanders SL, Romier C, Kirschner D, Weil PA, Tora L, Davidson I

We show that the yeast TFIID (yTFIID) component yTAF(II)47 contains a histone fold domain (HFD) with homology to that previously described for hTAF(II)135. Complementation in vivo indicates that the yTAF(II)47 HFD is necessary and sufficient for vegetative growth. Mutation of highly conserved residues in the alpha1 helix of the yTAF(II)47 HFD results in a temperature-sensitive phenotype which can be suppressed ... [more]

Mol. Cell. Biol. Mar. 01, 2001; 21(5);1841-53 [Pubmed: 11238921]

Throughput

Low Throughput

Ontology Terms

phenotype: heat sensitivity (APO:0000147)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TAF13 TAF3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Auty R (2004)	High	-	BioGRID	147061
TAF13 TAF3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Babu M (2012)	High	-	BioGRID	694740
TAF13 TAF3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2002)	High	-	BioGRID	-
TAF13 TAF3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	6	BioGRID	3617474
TAF13 TAF3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Sanders SL (2002)	High	-	BioGRID	-
TAF3 TAF13	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Sanders SL (2002)	High	-	BioGRID	-
TAF3 TAF13	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.5317	BioGRID	1954613

Curated By

BioGRID

Interaction Summary

TAF3

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]chromatin binding [IDA]

Gene Ontology Cellular Component

TAF13

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]

Gene Ontology Cellular Component

Dosage Rescue

Publication

Histone folds mediate selective heterodimerization of yeast TAF(II)25 with TFIID components yTAF(II)47 and yTAF(II)65 and with SAGA component ySPT7.

Throughput

Ontology Terms

Related interactions

Curated By

RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]
chromatin binding [IDA]