MEC3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RFA1
Gene Ontology Biological Process
- DNA repair [IMP]
- DNA replication [IMP]
- DNA topological change [IDA]
- DNA unwinding involved in DNA replication [IDA]
- double-strand break repair via homologous recombination [IGI]
- establishment of protein localization [IPI]
- heteroduplex formation [IDA]
- mitotic recombination [IMP]
- nucleotide-excision repair [IDA]
- protein ubiquitination [IMP, IPI]
- reciprocal meiotic recombination [IMP]
- telomere maintenance via recombination [IGI]
- telomere maintenance via telomerase [IPI]
- telomere maintenance via telomere lengthening [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Synthetic Growth Defect
A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.
Publication
Yeast pip3/mec3 mutants fail to delay entry into S phase and to slow DNA replication in response to DNA damage, and they define a functional link between Mec3 and DNA primase.
The catalytic DNA primase subunit of the DNA polymerase alpha-primase complex is encoded by the essential PRI1 gene in Saccharomyces cerevisiae. To identify factors that functionally interact with yeast DNA primase in living cells, we developed a genetic screen for mutants that are lethal at the permissive temperature in a cold-sensitive pril-2 genetic background. Twenty-four recessive mutations belonging to seven ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: vegetative growth (APO:0000106)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MEC3 RFA1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.3281 | BioGRID | 2058139 | |
| MEC3 RFA1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.2976 | BioGRID | 2439343 | |
| MEC3 RFA1 | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 810411 |
Curated By
- BioGRID