BAIT

TAH11

CDT1, SID2, L000004733, YJR046W
DNA replication licensing factor; required for pre-replication complex assembly
Saccharomyces cerevisiae (S288c)
PREY

TOP1

MAK1, MAK17, DNA topoisomerase 1, L000002319, YOL006C
Topoisomerase I; nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination; role in processing ribonucleoside monophosphates in genomic DNA into irreversible single-strand breaks
Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Interdependent nuclear accumulation of budding yeast Cdt1 and Mcm2-7 during G1 phase.

Tanaka S, Diffley JF

Cdt1 is essential for loading Mcm2-7 proteins into prereplicative complexes (pre-RCs) during replication licensing and has been found in organisms as diverse as fission yeast and humans. We have identified a homologue of Cdt1 in Saccharomyces cerevisiae, which is required for pre-RC assembly. We show that, like Mcm2-7p, Cdt1p accumulates in the nucleus during G1 phase and is excluded from ... [more]

Nat. Cell Biol. Mar. 01, 2002; 4(3);198-207 [Pubmed: 11836525]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Curated By

  • BioGRID