BAIT
RAT1
HKE1, TAP1, XRN2, ssRNA exonuclease RAT1, L000001584, YOR048C
Nuclear 5' to 3' single-stranded RNA exonuclease; involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination; required for cotranscriptional pre-rRNA cleavage
GO Process (6)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
MOT1
BUR3, END10, LPF4, BTAF1, L000001136, L000004559, S000029158, YPL082C
Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity; may have a role in ensuring that soluble TBP is available to bind TATA-less promoters; forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA
GO Process (5)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- negative regulation of transcription, DNA-templated [IMP]
- rRNA processing [IMP]
- regulation of RNA polymerase II transcriptional preinitiation complex assembly [IMP]
- regulation of transcription from RNA polymerase II promoter [IDA]
- transcription of nuclear large rRNA transcript from RNA polymerase I promoter [IDA, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
An early function during transcription for the yeast mRNA export factor Dbp5p/Rat8p suggested by its genetic and physical interactions with transcription factor IIH components.
The yeast DEAD-box protein Dbp5p/Rat8p is an essential factor for mRNA export and shuttles between the nucleus and the cytoplasm. It is concentrated at the cytoplasmic fibrils of the nuclear pore complex where it interacts with several nucleoporins. On the basis of this localization, it has been suggested that it might participate in a terminal step of RNA export, the ... [more]
Mol. Biol. Cell Apr. 01, 2003; 14(4);1664-76 [Pubmed: 12686617]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID