A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

The Rpb9 subunit of RNA polymerase II binds transcription factor TFIIE and interferes with the SAGA and elongator histone acetyltransferases.

Van Mullem V, Wery M, Werner M, Vandenhaute J, Thuriaux P

Rpb9 is a small subunit of yeast RNA polymerase II participating in elongation and formed of two conserved zinc domains. rpb9 mutants are viable, with a strong sensitivity to nucleotide-depleting drugs. Deleting the C-terminal domain down to the first 57 amino acids has no detectable growth defect. Thus, the critical part of Rpb9 is limited to a N-terminal half that ... [more]

J. Biol. Chem. Mar. 22, 2002; 277(12);10220-5 [Pubmed: 11779853]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RPB9 TFA1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Van Mullem V (2002)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RPB9

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA-directed RNA polymerase activity [IDA]

Gene Ontology Cellular Component

TFA1