BAIT

SEC9

HSS7, L000001835, YGR009C
t-SNARE protein required for secretory vesicle-plasma membrane fusion; similar to but not functionally redundant with Spo20p; interacts non-exocyst bound Sec6p; SNAP-25 homolog
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

MSB3

GYP3, L000003918, YNL293W
Rab GTPase-activating protein; regulates endocytosis via inactivation of Vps21p at endosomes and vacuole fusion via inactivation of Ypt7p at vacuoles; also acts on Ypt52p and Sec4p; required for proper actin organization; also localizes to plasma membrane and sites of polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; similar to the TBC-domain Tre2 oncogene; MSB3 has a paralog, MSB4, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

The GAP activity of Msb3p and Msb4p for the Rab GTPase Sec4p is required for efficient exocytosis and actin organization.

Gao XD, Albert S, Tcheperegine SE, Burd CG, Gallwitz D, Bi E

Polarized growth in Saccharomyces cerevisiae is thought to occur by the transport of post-Golgi vesicles along actin cables to the daughter cell, and the subsequent fusion of the vesicles with the plasma membrane. Previously, we have shown that Msb3p and Msb4p genetically interact with Cdc42p and display a GTPase-activating protein (GAP) activity toward a number of Rab GTPases in vitro. ... [more]

J. Cell Biol. Aug. 18, 2003; 162(4);635-46 [Pubmed: 12913108]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: heat sensitivity (APO:0000147)
  • phenotype: inviable (APO:0000112)

Additional Notes

  • deletion of msb3 and msb4 causes lethality in a sec9 mutant
  • genetic complex
  • in msb4 background

Curated By

  • BioGRID