BAIT

SIT1

ARN3, YEL065W
Ferrioxamine B transporter; member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p
GO Process (3)
GO Function (1)
GO Component (3)
Saccharomyces cerevisiae (S288c)
PREY

DST1

PPR2, SII, S-II, TFIIS, P37, L000001476, L000000530, YGL043W
General transcription elongation factor TFIIS; enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress
Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Stimulation of transcription by mutations affecting conserved regions of RNA polymerase II.

Archambault J, Jansma DB, Kawasoe JH, Arndt KT, Greenblatt J, Friesen JD

Mutations that increase the low-level transcription of the Saccharomyces cerevisiae HIS4 gene, which results from deletion of the genes encoding transcription factors BAS1, BAS2, and GCN4, were isolated previously in SIT1 (also known as RPO21, RPB1, and SUA8), the gene encoding the largest subunit of RNA polymerase II (RNAPII). Here we show that sit1 substitutions cluster in two conserved regions ... [more]

J. Bacteriol. May. 01, 1998; 180(10);2590-8 [Pubmed: 9573141]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Curated By

  • BioGRID