BAIT
CSL4
SKI4, L000001905, L000004677, YNL232W
Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
GO Process (10)
GO Function (0)
GO Component (4)
Gene Ontology Biological Process
- exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [IMP]
- ncRNA 3'-end processing [IC]
- nonfunctional rRNA decay [IC]
- nuclear polyadenylation-dependent mRNA catabolic process [IC]
- nuclear polyadenylation-dependent rRNA catabolic process [IMP]
- nuclear polyadenylation-dependent tRNA catabolic process [IDA]
- nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [IC]
- nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' [IGI, IMP]
- nuclear-transcribed mRNA catabolic process, non-stop decay [IMP]
- polyadenylation-dependent snoRNA 3'-end processing [IC]
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
DCP2
PSU1, decapping enzyme complex catalytic subunit DCP1, L000002866, YNL118C
Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; also enters the nucleus and positively regulates transcription initiation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress
GO Process (4)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Function of the ski4p (Csl4p) and Ski7p proteins in 3'-to-5' degradation of mRNA.
One of two general pathways of mRNA decay in the yeast Saccharomyces cerevisiae occurs by deadenylation followed by 3'-to-5' degradation of the mRNA body. Previous results have shown that this degradation requires components of the exosome and the Ski2p, Ski3p, and Ski8p proteins, which were originally identified due to their superkiller phenotype. In this work, we demonstrate that deletion of ... [more]
Mol. Cell. Biol. Nov. 01, 2000; 20(21);8230-43 [Pubmed: 11027292]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID