BAIT
SKP1
MGO1, SCF ubiquitin ligase subunit SKP1, CBF3D, L000001910, YDR328C
Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
GO Process (12)
GO Function (4)
GO Component (6)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IMP]
- G2/M transition of mitotic cell cycle [IMP]
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [IDA]
- exit from mitosis [IMP]
- kinetochore assembly [IDA, IMP]
- protein complex assembly [IDA]
- protein neddylation [IGI, IMP]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA]
- regulation of exit from mitosis [IMP, IPI]
- regulation of protein complex assembly [IPI]
- septin ring assembly [IMP]
- vacuolar acidification [IGI, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
MAD2
spindle checkpoint protein MAD2, L000000975, YJL030W
Component of the spindle-assembly checkpoint complex; delays onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability
GO Process (3)
GO Function (0)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
The spindle checkpoint of the yeast Saccharomyces cerevisiae requires kinetochore function and maps to the CBF3 domain.
We have measured the activity of the spindle checkpoint in null mutants lacking kinetochore activity in the yeast Saccharomyces cerevisiae. We constructed deletion mutants for nonessential genes by one-step gene replacements. We constructed heterozygous deletions of one copy of essential genes in diploid cells and purified spores containing the deletion allele. In addition, we made gene fusions for three essential ... [more]
Genetics Apr. 01, 2001; 157(4);1493-502 [Pubmed: 11290706]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID