BAIT
SPT4
transcription elongation factor SPT4, L000002030, YGR063C
Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions
GO Process (12)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
- 7-methylguanosine mRNA capping [IGI]
- chromatin organization [IMP]
- chromatin silencing [IMP]
- chromosome segregation [IMP]
- intracellular mRNA localization [IMP]
- mRNA splicing, via spliceosome [IMP]
- negative regulation of transcription elongation from RNA polymerase I promoter [IGI]
- positive regulation of transcription elongation from RNA polymerase I promoter [IMP]
- positive regulation of transcription elongation from RNA polymerase II promoter [IMP]
- regulation of rRNA processing [IMP]
- regulation of transcription, DNA-templated [IMP]
- regulation of transcription-coupled nucleotide-excision repair [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
THI2
PHO6, phoF, L000003526, YBR240C
Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type
GO Process (2)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Molecular evidence for a positive role of Spt4 in transcription elongation.
We have previously shown that yeast mutants of the THO complex have a defect in gene expression, observed as an impairment of lacZ transcription. Here we analyze the ability of mutants of different transcription elongation factors to transcribe lacZ. We found that spt4Delta, like THO mutants, impaired transcription of lacZ and of long and GC-rich DNA sequences fused to the ... [more]
EMBO J. Feb. 03, 2003; 22(3);612-20 [Pubmed: 12554661]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID