BAIT

VPS21

VPS12, VPT12, YPT21, YPT51, Rab family GTPase VPS21, L000002474, YOR089C
Endosomal Rab family GTPase; required for endocytic transport and sorting of vacuolar hydrolases; required for endosomal localization of the CORVET complex; required with YPT52 for MVB biogenesis and sorting; involved in autophagy and ionic stress tolerance; geranylgeranylation required for membrane association; protein abundance increases in response to DNA replication stress; mammalian Rab5 homolog; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

VAN1

LDB13, VRG7, VRG8, L000002453, YML115C
Component of the mannan polymerase I; complex contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Use of a synthetic lethal screen to identify yeast mutants impaired in endocytosis, vacuolar protein sorting and the organization of the cytoskeleton.

Singer-Krueger B, Ferro-Novick S

To identify new genes whose products act on the endocytic and vacuolar protein sorting pathways, we screened for mutants that display synthetic growth defects with delta ypt51, a mutant impaired in membrane traffic at a point where these pathways intersect. Seven mutants that fell into six different complementation groups were found to fit this criterium. Two of the mutants (ysl1 ... [more]

Eur. J. Cell Biol. Dec. 01, 1997; 74(4);365-75 [Pubmed: 9438133]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
VAN1 VPS21
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1564BioGRID
2160074

Curated By

  • BioGRID