BAIT
MSL5
BBP, L000004109, YLR116W
Component of commitment complex; which defines first step in splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA; relocalizes to the cytosol in response to hypoxia
GO Process (1)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
NAM7
IFS2, MOF4, SUP113, UPF1, ATP-dependent RNA helicase NAM7, L000002429, S000029550, L000002232, YMR080C
ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; binds to the small ribosomal subunit via an interaction with Rps26; forms cytoplasmic foci upon DNA replication stress
GO Process (9)
GO Function (5)
GO Component (3)
Gene Ontology Biological Process
- DNA recombination [IMP]
- chromatin silencing at silent mating-type cassette [IGI]
- intracellular mRNA localization [IMP]
- nuclear-transcribed mRNA catabolic process [IMP]
- nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [IGI, IMP]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [TAS]
- protein ubiquitination [IDA, IMP, IPI]
- regulation of translational termination [TAS]
- translational frameshifting [IMP]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
A dual role for BBP/ScSF1 in nuclear pre-mRNA retention and splicing.
The MSL5 gene, which codes for the splicing factor BBP/ScSF1, is essential in Saccharomyces cerevisiae, yet previous analyses failed to reveal a defect in assembly of (pre)-spliceosomes or in vitro splicing associated with its depletion. We generated 11 temperature-sensitive (ts) mutants and one cold-sensitive (cs) mutant in the corresponding gene and analyzed their phenotypes. While all mutants were blocked in ... [more]
EMBO J. Apr. 17, 2000; 19(8);1873-86 [Pubmed: 10775271]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID