BAIT

LAC1

DGT1, sphingosine N-acyltransferase LAC1, YKL008C
Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p; LAC1 has a paralog, LAG1, that arose from the whole genome duplication
GO Process (2)
GO Function (1)
GO Component (3)
Saccharomyces cerevisiae (S288c)
PREY

YPC1

phytoceramidase, YBR183W
Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication
GO Process (2)
GO Function (2)
GO Component (3)
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Lag1p and Lac1p are essential for the Acyl-CoA-dependent ceramide synthase reaction in Saccharomyces cerevisae.

Schorling S, Vallee B, Barz WP, Riezman H, Oesterhelt D

Lag1p and Lac1p are two homologous transmembrane proteins of the endoplasmic reticulum in Saccharomyces cerevisiae. Homologous genes have been found in a wide variety of eukaryotes. In yeast, both genes, LAC1 and LAG1, are required for efficient endoplasmic reticulum-to-Golgi transport of glycosylphosphatidylinositol-anchored proteins. In this study, we show that lag1 Delta lac1 Delta cells have reduced sphingolipid levels due to ... [more]

Mol. Biol. Cell Nov. 01, 2001; 12(11);3417-27 [Pubmed: 11694577]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: metabolism and growth (APO:0000094)
  • phenotype: viability (APO:0000111)
  • phenotype: resistance to chemicals (APO:0000087)

Additional Notes

  • lac1 lag1 ypc1 ydc1 quadruple mutant has extra defects

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
LAC1 YPC1
Dosage Rescue
Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Low-BioGRID
162149
LAC1 YPC1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
521986

Curated By

  • BioGRID