BAIT

RPC10

RPB12, DNA-directed RNA polymerase core subunit RPB12, ABC10-alpha, L000001686, YHR143W-A
RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

RPB2

RPB150, RPO22, SIT2, SOH2, DNA-directed RNA polymerase II core subunit RPB2, B150, L000001588, L000001676, YOR151C
RNA polymerase II second largest subunit B150; part of central core; similar to bacterial beta subunit
GO Process (1)
GO Function (3)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Lethality

A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.

Publication

Functional characterization of ABC10alpha, an essential polypeptide shared by all three forms of eukaryotic DNA-dependent RNA polymerases.

Rubbi L, Labarre-Mariotte S, Chedin S, Thuriaux P

ABC10alpha is a small polypeptide shared by the three yeast RNA polymerases. Homologous polypeptides in higher eukaryotes have a zinc-binding CX(2)C. CX(2)C motif and a conserved basic C-terminal end. These features are also found in archaeal gene products that may encode an RNA polymerase subunit. The CX(2)C. CX(2)C motif is partly dispensable, since only its first cysteine is essential for ... [more]

J. Biol. Chem. Oct. 29, 1999; 274(44);31485-92 [Pubmed: 10531351]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RPC10 RPB2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High6BioGRID
3612289

Curated By

  • BioGRID