BAIT

RPB9

SSU73, DNA-directed RNA polymerase II core subunit RPB9, B12.6, SHI, L000001683, L000001880, YGL070C
RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription
Saccharomyces cerevisiae (S288c)
PREY

SPT7

GIT2, SAGA histone acetyltransferase complex subunit SPT7, L000002033, YBR081C
Subunit of the SAGA transcriptional regulatory complex; involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex
GO Process (4)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Members of the SAGA and Mediator complexes are partners of the transcription elongation factor TFIIS.

Wery M, Shematorova E, Van Driessche B, Vandenhaute J, Thuriaux P, Van Mullem V

TFIIS, an elongation factor encoded by DST1 in Saccharomyces cerevisiae, stimulates transcript cleavage in arrested RNA polymerase II. Two components of the RNA polymerase II machinery, Med13 (Srb9) and Spt8, were isolated as two-hybrid partners of the conserved TFIIS N-terminal domain. They belong to the Cdk8 module of the Mediator and to a subform of the SAGA co-activator, respectively. Co-immunoprecipitation ... [more]

EMBO J. Oct. 27, 2004; 23(21);4232-42 [Pubmed: 15359273]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Curated By

  • BioGRID