BAIT

GAS1

CWH52, GGP1, 1,3-beta-glucanosyltransferase GAS1, L000000669, YMR307W
Beta-1,3-glucanosyltransferase; required for cell wall assembly and also has a role in transcriptional silencing; localizes to cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at nuclear periphery; genetic interactions with histone H3 lysine acetyltransferases GCN5 and SAS3 indicate previously unsuspected functions for Gas1 in DNA damage response and cell cycle regulation
Saccharomyces cerevisiae (S288c)
PREY

KEX2

QDS1, SRB1, VMA45, kexin KEX2, yscF, L000000896, YNL238W
Kexin, a subtilisin-like protease (proprotein convertase); a calcium-dependent serine protease involved in the activation of proproteins of the secretory pathway
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

SKG1, a suppressor gene of synthetic lethality of kex2Deltagas1Delta mutations, encodes a novel membrane protein that affects cell wall composition.

Tomishige N, Noda Y, Adachi H, Shimoi H, Yoda K

The fungal GAS1-related genes encode GPI-anchored beta-1,3-glucanosyltransferase, and their loss causes a defect in the assembly of the cell wall. The KEX2 gene encodes a processing protease in the late Golgi compartment and its loss also results in defects in the cell wall. Simultaneous mutations of these genes are lethal in Saccharomyces cerevisiae. To understand the basis of this synthetic ... [more]

Yeast Jan. 30, 2005; 22(2);141-55 [Pubmed: 15645486]

Throughput

  • Low Throughput

Ontology Terms

  • viability (APO:0000111)

Additional Notes

  • kex2 gas1 background

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GAS1 KEX2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-16.7966BioGRID
898191
GAS1 KEX2
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
644881

Curated By

  • BioGRID