BAIT
NSE1
YLR007W
Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
GO Process (2)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
MLP1
MPL1, L000001141, L000001122, YKR095W
Myosin-like protein associated with the nuclear envelope; nuclear basket protein that connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs; MLP1 has a paralog, MLP2, that arose from the whole genome duplication
GO Process (7)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
- negative regulation of protein import into nucleus during spindle assembly checkpoint [IGI]
- nuclear retention of unspliced pre-mRNA at the site of transcription [IGI, IMP]
- poly(A)+ mRNA export from nucleus [IMP]
- protein import into nucleus [IGI]
- protein localization to nuclear pore [IMP]
- telomere tethering at nuclear periphery [IGI]
- transcriptional activation by promoter-terminator looping [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization.
Through a genetic screen using myosin-like protein strains mlp1Delta mlp2Delta and biochemical purification, we identified a complex of eight proteins, each required for growth and DNA repair in Saccharomyces cerevisiae. Among the subunits are Mms21 that contains a putative Siz/PIAS (protein inhibitor of activated signal transducer and activator of transcription) RING domain characteristic of small ubiquitin-like modifier (SUMO) ligases, two ... [more]
Proc. Natl. Acad. Sci. U.S.A. Mar. 29, 2005; 102(13);4777-82 [Pubmed: 15738391]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Additional Notes
- nse1 mlp1 mlp2 triple mutant
Curated By
- BioGRID