BAIT
DAM1
L000004420, YGR113W
Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Duo1p to connect the DASH complex with the microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments
GO Process (3)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
RAS2
CTN5, CYR3, GLC5, TSL7, Ras family GTPase RAS2, L000001583, YNL098C
GTP-binding protein; regulates nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes; RAS2 has a paralog, RAS1, that arose from the whole genome duplication
GO Process (8)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
- activation of adenylate cyclase activity [IDA]
- ascospore formation [IMP]
- positive regulation of adenylate cyclase activity [IGI]
- positive regulation of pseudohyphal growth [IMP]
- positive regulation of transcription by galactose [IMP]
- protein localization to bud neck [IGI]
- regulation of protein localization [IMP]
- replicative cell aging [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Genetic analysis of the kinetochore DASH complex reveals an antagonistic relationship with the ras/protein kinase A pathway and a novel subunit required for Ask1 association.
DASH is a microtubule- and kinetochore-associated complex required for proper chromosome segregation and bipolar attachment of sister chromatids on the mitotic spindle. We have undertaken a genetic and biochemical analysis of the DASH complex and uncovered a strong genetic interaction of DASH with the Ras/protein kinase A (PKA) pathway. Overexpression of PDE2 or deletion of RAS2 rescued the temperature sensitivity ... [more]
Mol. Cell. Biol. Jan. 01, 2005; 25(2);767-78 [Pubmed: 15632076]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Related interactions
Curated By
- BioGRID