BAIT
RAD6
PSO8, UBC2, E2 ubiquitin-conjugating protein RAD6, L000001560, YGL058W
Ubiquitin-conjugating enzyme (E2); involved in postreplication repair as a heterodimer with Rad18p, DSBR and checkpoint control as a heterodimer with Bre1p, ubiquitin-mediated N-end rule protein degradation as a heterodimer with Ubr1p, as well as endoplasmic reticulum-associated protein degradation (ERAD) with Ubr1p in the absence of canonical ER membrane ligases
GO Process (17)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
- DNA-templated transcription, termination [IMP]
- ER-associated ubiquitin-dependent protein catabolic process [IGI]
- chromatin silencing at telomere [IMP]
- double-strand break repair via homologous recombination [IGI]
- error-free postreplication DNA repair [IGI]
- error-free translesion synthesis [IGI]
- error-prone translesion synthesis [IGI]
- histone monoubiquitination [IMP]
- meiotic DNA double-strand break formation [IMP]
- mitotic G1 DNA damage checkpoint [IMP]
- protein monoubiquitination [IMP]
- protein polyubiquitination [IMP]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IMP]
- regulation of dipeptide transport [IMP]
- telomere maintenance via recombination [IGI]
- transcription from RNA polymerase II promoter [IPI]
- ubiquitin-dependent protein catabolic process via the N-end rule pathway [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
RPB9
SSU73, DNA-directed RNA polymerase II core subunit RPB9, B12.6, SHI, L000001683, L000001880, YGL070C
RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription
GO Process (5)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
- cellular response to DNA damage stimulus [IMP]
- maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase II promoter [IGI, IMP]
- transcription from RNA polymerase II promoter [IMP]
- transcription initiation from RNA polymerase II promoter [IMP]
- transcription-coupled nucleotide-excision repair [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Histone H2B ubiquitylation is associated with elongating RNA polymerase II.
Rad6-mediated ubiquitylation of histone H2B at lysine 123 has been linked to transcriptional activation and the regulation of lysine methylation on histone H3. However, how Rad6 and H2B ubiquitylation contribute to the transcription and histone methylation processes is poorly understood. Here, we show that the Paf1 transcription elongation complex and the E3 ligase for Rad6, Bre1, mediate an association of ... [more]
Mol. Cell. Biol. Jan. 01, 2005; 25(2);637-51 [Pubmed: 15632065]
Throughput
- High Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID